Профессиональные интересы/научные проекты:
Лаборатория исследует мембранные рецепторы и ионные каналы, а также соединения, действующие на них (токсины, эндогенные лиганды). Для исследования структуры и механизмов действия на молекулярном уровне мы применяем современные методы структурной биологии: ЯМР-спектроскопию и криоэлектронную микроскопию. В настоящее время мы изучаем механизмы взаимодействия токсинов с потенциал-зависимыми K+ и Na+ каналами (Kv и Nav), а также исследуем структуры белковых лигандов никотинового ацетилхолинового рецептора (nAChR). Структурные исследования мембранных рецепторов и каналов необходимы для разработки новых средств диагностики и терапии заболеваний нервной, сердечно-сосудистой и мышечной систем организма. Кроме того, мы исследуем структуры и механизмы действия антибиотиков и защитных пептидов растений и животных. Эти исследования необходимы для создания новых препаратов для лечения инфекционных заболеваний.
Основные публикации:
1.Shulepko MA, Bychkov ML, Shenkarev ZO, Kulbatskii DS, Makhonin AM, Paramonov AS, Chugunov AO, Kirpichnikov MP, Lyukmanova EN. Biochemical Basis of Skin Disease Mal de Meleda: SLURP-1 Mutants Differently Affect Keratinocyte Proliferation and Apoptosis. J Invest Dermatol. 2021 Sep;141(9):2229-2237. doi: 10.1016/j.jid.2021.01.035. Epub 2021 Mar 16. PMID: 33741389.
2.Tyurin AP, Alferova VA, Paramonov AS, Shuvalov MV, Kudryakova GK, Rogozhin EA, Zherebker AY, Brylev VA, Chistov AA, Baranova AA, Biryukov MV, Ivanov IA, Prokhorenko IA, Grammatikova NE, Kravchenko TV, Isakova EB, Mirchink EP, Gladkikh EG, Svirshchevskaya EV, Mardanov AV, Beletsky AV, Kocharovskaya MV, Kulyaeva VV, Shashkov AS, Tsvetkov DE, Nifantiev NE, Apt AS, Majorov KB, Efimova SS, Ravin NV, Nikolaev EN, Ostroumova OS, Katrukha GS, Lapchinskaya OA, Dontsova OA, Terekhov SS, Osterman IA, Shenkarev ZO, Korshun VA. Gausemycins A,B: Cyclic Lipoglycopeptides from Streptomyces sp.*. Angew Chem Int Ed Engl. 2021 Aug 16;60(34):18694-18703. doi: 10.1002/anie.202104528. Epub 2021 Jun 22. PMID: 34009717.
3.Bolosov IA, Panteleev PV, Sychev SV, Sukhanov SV, Mironov PA, Myshkin MY, Shenkarev ZO, Ovchinnikova TV. Dodecapeptide Cathelicidins of Cetartiodactyla: Structure, Mechanism of Antimicrobial Action, and Synergistic Interaction With Other Cathelicidins. Front Microbiol. 2021 Aug 13;12:725526. doi: 10.3389/fmicb.2021.725526. PMID: 34484167; PMCID: PMC8415029.
4.Paramonov AS, Kocharovskaya MV, Tsarev AV, Kulbatskii DS, Loktyushov EV, Shulepko MA, Kirpichnikov MP, Lyukmanova EN, Shenkarev ZO. Structural Diversity and Dynamics of Human Three-Finger Proteins Acting on Nicotinic Acetylcholine Receptors. Int J Mol Sci. 2020 Oct 1;21(19):7280. doi: 10.3390/ijms21197280. PMID: 33019770; PMCID: PMC7582953.
5.Shenkarev ZO, Shulepko MA, Bychkov ML, Kulbatskii DS, Shlepova OV, Vasilyeva NA, Andreev-Andrievskiy AA, Popova AS, Lagereva EA, Loktyushov EV, Koshelev SG, Thomsen MS, Dolgikh DA, Kozlov SA, Balaban PM, Kirpichnikov MP, Lyukmanova EN. Water-soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7-nAChR dysfunction. J Neurochem. 2020 Oct;155(1):45-61. doi: 10.1111/jnc.15018. Epub 2020 May 10. PMID: 32222974.
6.Myshkin MY, Männikkö R, Krumkacheva OA, Kulbatskii DS, Chugunov AO, Berkut AA, Paramonov AS, Shulepko MA, Fedin MV, Hanna MG, Kullmann DM, Bagryanskaya EG, Arseniev AS, Kirpichnikov MP, Lyukmanova EN, Vassilevski AA, Shenkarev ZO. Cell-Free Expression of Sodium Channel Domains for Pharmacology Studies. Noncanonical Spider Toxin Binding Site in the Second Voltage-Sensing Domain of Human Nav1.4 Channel. Front Pharmacol. 2019 Sep 4;10:953. doi: 10.3389/fphar.2019.00953. PMID: 31555136; PMCID: PMC6737007.
7.Männikkö R, Shenkarev ZO, Thor MG, Berkut AA, Myshkin MY, Paramonov AS, Kulbatskii DS, Kuzmin DA, Sampedro Castañeda M, King L, Wilson ER, Lyukmanova EN, Kirpichnikov MP, Schorge S, Bosmans F, Hanna MG, Kullmann DM, Vassilevski AA. Spider toxin inhibits gating pore currents underlying periodic paralysis. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4495-4500. doi: 10.1073/pnas.1720185115. Epub 2018 Apr 10. PMID: 29636418; PMCID: PMC5924911.
2.Tyurin AP, Alferova VA, Paramonov AS, Shuvalov MV, Kudryakova GK, Rogozhin EA, Zherebker AY, Brylev VA, Chistov AA, Baranova AA, Biryukov MV, Ivanov IA, Prokhorenko IA, Grammatikova NE, Kravchenko TV, Isakova EB, Mirchink EP, Gladkikh EG, Svirshchevskaya EV, Mardanov AV, Beletsky AV, Kocharovskaya MV, Kulyaeva VV, Shashkov AS, Tsvetkov DE, Nifantiev NE, Apt AS, Majorov KB, Efimova SS, Ravin NV, Nikolaev EN, Ostroumova OS, Katrukha GS, Lapchinskaya OA, Dontsova OA, Terekhov SS, Osterman IA, Shenkarev ZO, Korshun VA. Gausemycins A,B: Cyclic Lipoglycopeptides from Streptomyces sp.*. Angew Chem Int Ed Engl. 2021 Aug 16;60(34):18694-18703. doi: 10.1002/anie.202104528. Epub 2021 Jun 22. PMID: 34009717.
3.Bolosov IA, Panteleev PV, Sychev SV, Sukhanov SV, Mironov PA, Myshkin MY, Shenkarev ZO, Ovchinnikova TV. Dodecapeptide Cathelicidins of Cetartiodactyla: Structure, Mechanism of Antimicrobial Action, and Synergistic Interaction With Other Cathelicidins. Front Microbiol. 2021 Aug 13;12:725526. doi: 10.3389/fmicb.2021.725526. PMID: 34484167; PMCID: PMC8415029.
4.Paramonov AS, Kocharovskaya MV, Tsarev AV, Kulbatskii DS, Loktyushov EV, Shulepko MA, Kirpichnikov MP, Lyukmanova EN, Shenkarev ZO. Structural Diversity and Dynamics of Human Three-Finger Proteins Acting on Nicotinic Acetylcholine Receptors. Int J Mol Sci. 2020 Oct 1;21(19):7280. doi: 10.3390/ijms21197280. PMID: 33019770; PMCID: PMC7582953.
5.Shenkarev ZO, Shulepko MA, Bychkov ML, Kulbatskii DS, Shlepova OV, Vasilyeva NA, Andreev-Andrievskiy AA, Popova AS, Lagereva EA, Loktyushov EV, Koshelev SG, Thomsen MS, Dolgikh DA, Kozlov SA, Balaban PM, Kirpichnikov MP, Lyukmanova EN. Water-soluble variant of human Lynx1 positively modulates synaptic plasticity and ameliorates cognitive impairment associated with α7-nAChR dysfunction. J Neurochem. 2020 Oct;155(1):45-61. doi: 10.1111/jnc.15018. Epub 2020 May 10. PMID: 32222974.
6.Myshkin MY, Männikkö R, Krumkacheva OA, Kulbatskii DS, Chugunov AO, Berkut AA, Paramonov AS, Shulepko MA, Fedin MV, Hanna MG, Kullmann DM, Bagryanskaya EG, Arseniev AS, Kirpichnikov MP, Lyukmanova EN, Vassilevski AA, Shenkarev ZO. Cell-Free Expression of Sodium Channel Domains for Pharmacology Studies. Noncanonical Spider Toxin Binding Site in the Second Voltage-Sensing Domain of Human Nav1.4 Channel. Front Pharmacol. 2019 Sep 4;10:953. doi: 10.3389/fphar.2019.00953. PMID: 31555136; PMCID: PMC6737007.
7.Männikkö R, Shenkarev ZO, Thor MG, Berkut AA, Myshkin MY, Paramonov AS, Kulbatskii DS, Kuzmin DA, Sampedro Castañeda M, King L, Wilson ER, Lyukmanova EN, Kirpichnikov MP, Schorge S, Bosmans F, Hanna MG, Kullmann DM, Vassilevski AA. Spider toxin inhibits gating pore currents underlying periodic paralysis. Proc Natl Acad Sci U S A. 2018 Apr 24;115(17):4495-4500. doi: 10.1073/pnas.1720185115. Epub 2018 Apr 10. PMID: 29636418; PMCID: PMC5924911.
SPIN РИНЦ:
8799-1224
Researcher ID:
P-1808-2015
Scopus Author ID:
7801695075
ORCID:
0000-0003-1383-3522
Индекс Хирша:
26